Composite

Part:BBa_K3606079

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)


P1 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P1 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal XbaI site found at 6868
    Illegal SpeI site found at 5972
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal PstI site found at 5958
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5559
    Illegal NheI site found at 6930
    Illegal SpeI site found at 5972
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal PstI site found at 5958
    Illegal NotI site found at 5551
    Illegal NotI site found at 5963
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5559
    Illegal BamHI site found at 3378
    Illegal XhoI site found at 5709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal XbaI site found at 6868
    Illegal SpeI site found at 5972
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal PstI site found at 5958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5559
    Illegal XbaI site found at 5544
    Illegal XbaI site found at 6868
    Illegal SpeI site found at 5972
    Illegal PstI site found at 2382
    Illegal PstI site found at 2415
    Illegal PstI site found at 5958
    Illegal NgoMIV site found at 2044
    Illegal AgeI site found at 2216
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None